vimentin antibody (2d1)  (Bio-Techne corporation)

Journal: bioRxiv

Article Title: MUC2 Expression Modulates Immune Infiltration in Colorectal Cancer

doi: 10.1101/2024.08.06.594842

Figure Lengend Snippet: A. Box plot of adherence junction enrichment score (ES) in HT-29 and LS-174T. The ES is up-regulated in HT-29 MUC2 K.O. compared to CTRL (p=0.041) (student t-test). In contrast, the ES is down-regulated in LS-174T MUC2 K.O. compared to CTRL (p=0.047) (student t-test). B. Representative capillary western blot (CWB) results of E-cadherin, N-cadherin and Vimentin expression in human fetal fibroblast (HFF), Hela, HT-29 CTRL and HT-29 MUC2 K.O. (N=2 technical replicates). C. Representative capillary western blot (CWB) results of E-cadherin, N-cadherin and Vimentin expression in HFF, Hela, LS-174T CTRL and LS-174T MUC2 K.O. (N=2 technical replicates). D. Quantification of band intensity of E-cadherin, N-cadherin and Vimentin normalized to beta actin expression in HFF, Hela, HT-29 CTRL and HT-29 MUC2. (N=2, technical replicates). E. Quantification of band intensity of E-cadherin, N-cadherin and Vimentin normalized to beta actin expression in HFF, Hela, LS-174T CTRL and LS-174T MUC2 K.O.. (N=2, technical replicates).

Article Snippet: Mouse anti human E-Cadherin (R&D systems, #MAB1838) diluted at 0.5ug/ml, mouse anti human N-Cadherin (Novus Biologicals, #NBP1-48309) diluted at 1 in 10, mouse anti human Vimentin (Novus Biologicas, #NBP1-92687) diluted at 1 in 25 and anti β-actin (Licor, #926-42212) diluted at 1 in 100 were used as primary antibody.

Techniques: Western Blot, Expressing

Journal: International journal of molecular sciences

Article Title: Morphological Characterization of Human Lung Cancer Organoids Cultured in Type I Collagen Hydrogels: A Histological Approach.

doi: 10.3390/ijms241210131

Figure Lengend Snippet: Figure 4. Morphology of NFs (A–C) or CAFs (D–F) spheroids. Spheroids were constructed and cultured in type I collagen scaffolds for one week. F-actin distribution was analyzed via fluorescence staining with phalloidin-rhodamine (red). Vimentin expression was studied by immunofluorescence (green). DAPI stains nuclei blue. Samples were studied under an epifluorescence microscope. The results are representative of n = 3. Scale bar = 100 µm.

Article Snippet: The antibodies used were the primary monoclonal vimentin antibody (2D1; 1:500 dilution; Novus Biologicals, Englewood, CO, USA) and Pan-Alexa fluor cytokeratin (1:100 dilution; Invitrogen, Waltham, MA, USA).

Techniques: Construct, Cell Culture, Staining, Expressing, Microscopy

Journal: International journal of molecular sciences

Article Title: Morphological Characterization of Human Lung Cancer Organoids Cultured in Type I Collagen Hydrogels: A Histological Approach.

doi: 10.3390/ijms241210131

Figure Lengend Snippet: Figure 6. Morphology of A549 cells and CAFs organoids. Organoids were made by mixing A549 cells with 50% (A–C) or 70% (D,E) CAFs and cultured in type I collagen scaffolds for one week. F-actin distribution was analyzed via phalloidin-rhodamine fluorescence staining (red). Green tracer was used to specifically stain the keratin of A549 cells (green fluorescence, (B,F)). Pankeratin and vimentin expression was assayed by immunofluorescence staining ((C,H), respectively, green fluorescence). DAPI stains nuclei blue. Samples were studied under confocal microscopy. The depth of the organoids was estimated by confocal microscopy in the Z axis (D,G). The results are representative of n = 3. Scale bar = 100 µm.

Article Snippet: The antibodies used were the primary monoclonal vimentin antibody (2D1; 1:500 dilution; Novus Biologicals, Englewood, CO, USA) and Pan-Alexa fluor cytokeratin (1:100 dilution; Invitrogen, Waltham, MA, USA).

Techniques: Cell Culture, Staining, Expressing, Confocal Microscopy

Journal: International journal of molecular sciences

Article Title: Morphological Characterization of Human Lung Cancer Organoids Cultured in Type I Collagen Hydrogels: A Histological Approach.

doi: 10.3390/ijms241210131

Figure Lengend Snippet: Figure 10. Relative gene expression levels of CDH1 (A), VIM (B) and CDH2 (C). Spheroids/organoids were generated and cultured in type I collagen hydrogels for 72 h. Relative gene expression was calculated using real-time RT-PCR. Semi-comparative ∆∆Ct method was used to calculated relative changes. A549 cells spheroids was chosen as control group. The GAPDH gene was used as house- keeping gene. The mean ± SD of three independent experiments is represented. * p < 0.05 versus the control group. # p < 0.05 versus A549 cells + CAFs group.

Article Snippet: The antibodies used were the primary monoclonal vimentin antibody (2D1; 1:500 dilution; Novus Biologicals, Englewood, CO, USA) and Pan-Alexa fluor cytokeratin (1:100 dilution; Invitrogen, Waltham, MA, USA).

Techniques: Gene Expression, Generated, Cell Culture, Quantitative RT-PCR, Control